University of Pittsburgh Cancer Institute (UPCI)

CCSG Acknowledgement

Required CCSG Acknowledgement

The NCI requires that publications acknowledge the UPCI CCSG support, and they are tracking compliance. If a UPCI CCSG-supported Shared Facility provided data used in your publication, please include the following statement in the acknowledgment section of your publication(s):

"This project used the UPCI [insert name(s) of shared facility(ies)] that [is/are] supported in part by award P30CA047904."

Shared Facility Directors: Please make sure to include this statement on all of your order forms, contracts, etc. as a reminder to your users to acknowledge the UPCI CCSG support.


Before submitting...

  1. If you wish to submit samples to the CGF for both DNA/RNA extraction services and microarray services, you must first complete and submit this form for the extraction services only. Once the CGF has completed the extractions, verified sample quality, and sent you the data that contains your sample concentration, total volume, and total yield, you can use that information to submit a new request form for the microarray services.
  2. Nucleic acid samples must be submitted in 1.5 or 2.0 ml tubes.
  3. Names of the samples name must be clearly labeled on top of each tube and must exactly match names on the request form.
  4. All nucleic acid extractions conducted outside of the facility will be assessed for concentration on the Nanodrop and quality on the Bioanalyzer in the case of DNA. Investigators are responsible for the cost of this QC measure unless they can provide Nanodrop and Bioanalyzer files at the time of submission.
  5. Once your request form is received, you will receive a copy of the form in Adobe PDF format at the Contact Email address you supply. A member of the CGF will contact you to schedule sample drop-off.

CGF Laboratory Services Request Form

PI First &
Last Name:
PI E-mail:
Contact First
& Last Name:
Contact E-mail:
Contact Phone: Contact Fax:
Administrator
First and Last Name:
Administrator Phone:
Account Number:
Project Title:
Project Description:
1000 characters remaining

Service Requested

Please select at least one service for the samples.

DNA Extraction

DNA Qiagen
DNA Extraction with Deparaffinization
DNA RecoverALL – For FFPET Samples
DNA Flexigene – Extractions from Whole Blood
DNA Extraction – Organic – Not Recommended

RNA Extraction

RNA Qiagen - Recommended
RNA Qiagen – For FFPET Samples
RNA RecoverALL – For FFPET Samples
RNA Extraction – Organic – Not Recommended

Nucleic Acid Cleanup

DNA
RNA

Quality Analysis via Bioanalyzer

DNA
RNA

Quality Analysis via Bioanalyzer and Nanodrop - must submit elution buffer with samples

DNA
RNA

O.D. Quantitation via Nanodrop - must submit elution buffer with samples

DNA
RNA

Qubit Flourometric Quantitation

DNA
RNA

miRNA Expression

Affymetrix Assay
Exiqon Assay
TaqMan RT-PCR miRNA Assay
SA Biosciences RT-PCR miRNA Assay

RNA Expression

3' IVT Labeling Assays – For Affymetrix and Codelink 3' IVT Arrays
WT Expression Labeling Assay – For Affymetrix Exon and Gene 1.0 ST Arrays
TaqMan RT–PCR Expression Assay
Affymetrix Hybridization / Scan Only
Affymetrix Scan Only

DNA SNP Genotyping

Affymetrix 6.0
Affymetrix 5.0
Affymetrix 500K
Affymetrix 250K
Affymetrix 50K
Affymetrix 10K
TaqMan PCR Custom SNP Assay

DNA Sequencing

Whole Genome- SOLiD 5500
Exome- SOLid 5500
Ion AmpliSeq Comprehensive Cancer Panel
Ion AmpliSeq Cancer Hot Spot Panel
Ion AmpliSeq Custom Panel
Ion TargetSeq Custom Enrichment

RNA Sequencing

Small RNA AmpliSeq

Proteomics

Searchlight Protein Array Assay – Multiplex ELISA

Training Services

RNA Extraction
DNA Extraction
FFPET Extraction
Other - please specify below
Other service:

Fume Hood

Fume Hood Rental
How many samples will be submitted? (1 to 99)