UPMC Hillman Cancer Center

James M. Pipas, PhD

James Pipas
Herbert W. and Grace Boyer Chair in Molecular Biology
Professor of Biological Sciences


Contact Information

Contact Information

559B Crawford Hall
4249 Fifth Avenue
Pittsburgh, PA 15260

Phone: 412-624-4691
Email: pipas@pitt.edu

Research Keywords

Research Keywords

Simian virus 40 (SV40); T antigen; tumorigenesis

Research Summary

Research Summary

Simian virus 40 (SV40) belongs to a small collection of viruses that induce tumors. We utilize SV40 as a model system for understanding the molecular events that drive tumorigenesis. Our studies focus on the virus-encoded master regulatory protein, large T antigen. Large T antigen controls several aspects of viral infection including DNA replication, transcription and virion assembly. In addition, T antigen is necessary and, in most cases, sufficient for SV40-mediated tumorigenesis. T antigen induces tumors in rodents and the neoplastic transformation of cells in culture by binding to key cellular proteins that regulate proliferation and survival, and altering their activities. Our basic strategy is to use a combination of genetics and proteomics to identify cellular targets of T antigen and then to use molecular biology and mouse model approaches to understand how these actions contribute to tumorigenesis.

Selected Publications

Selected Publications

  • Liu H, Tang X, Srivastava A, Pécot T, Daniel P, Hemmelgarn B, Reyes S, Fackler N, Bajwa A, Kladney R, Koivisto C, Chen Z, Wang Q, Huang K, Machiraju R, Sáenz-Robles MT, Cantalupo P, Pipas JM, Leone G. Redeployment of Myc and E2f1-3 drives Rb-deficient cell cycles. Nat Cell Biol. 2015;17(8):1036-48. PubMed Link
  • Zhang H, Cockrell SK, Kolawole AO, Rotem A, Serohijos AW, Chang CB, Tao Y, Mehoke TS, Han Y, Lin JS, Giacobbi NS, Feldman AB, Shakhnovich E, Weitz DA, Wobus CE, Pipas JM. Isolation and analysis of rare norovirus recombinants from coinfected mice using drop-based microfluidics. J Virol. 2015;89(15):7722-34. PubMed Link
  • An P, Brodsky JL, Pipas JM. The conserved core enzymatic activities and the distinct dynamics of polyomavirus large T antigens. Arch Biochem Biophys. 2015;573:23-31. PubMed Link
  • Gupta T, Sáenz Robles MT, Pipas JM. Cellular transformation of mouse embryo fibroblasts in the absence of activator E2Fs. J Virol. 2015;89(9):5124-33. PubMed Link
  • Mishra N, Pereira M, Rhodes RH, An P, Pipas JM, Jain K, Kapoor A, Briese T, Faust PL, Lipkin WI. Identification of a novel polyomavirus in a pancreatic transplant recipient with retinal blindness and vasculitic myopathy. J Infect Dis. 2014;210(10):1595-9. PubMed Link
  • Katz JP, Pipas JM. SummonChimera infers integrated viral genomes with nucleotide precision from NGS data. BMC Bioinformatics. 2014;15:348. PubMed Link
  • Sáenz Robles MT, Chong JL, Koivisto C, Trimboli A, Liu H, Leone G, Pipas JM. Viral oncogene expression in the stem/progenitor cell compartment of the mouse intestine induces adenomatous polyps. Mol Cancer Res. 2014;12(10):1355-64. PubMed Link
  • Seneca NT, Sáenz Robles MT, Pipas JM. Removal of a small C-terminal region of JCV and SV40 large T antigens has differential effects on transformation. Virology. 2014;468-470:47-56. PubMed Link
  • Ireland AW, Gobillot TA, Gupta T, Seguin SP, Liang M, Resnick L, Goldberg MT, Manos-Turvey A, Pipas JM, Wipf P, Brodsky JL. Synthesis and structure–activity relationships of small molecule inhibitors of the simian virus 40 T antigen oncoprotein, an anti-polyomaviral target. Bioorg Med Chem. 2014;22(22):6490-502. PubMed Link
  • Forero A, Giacobbi NS, McCormick KD, Gjoerup OV, Bakkenist CJ, Pipas JM, Sarkar SN. Simian virus 40 large T antigen induces IFN-stimulated genes through ATR kinase. J Immunol. 2014;192(12):5933-42. PubMed Link

See all pubs (Pubmed)

Shared Resource Usage

Shared Resource Usage

  • Cell and Tissue Imaging Facility
  • Cytometry Facility

Department Website









Last modified: 8/20/15