UPMC Hillman Cancer Center

Yuan Chang, MD

Yuan Chang
Distinguished and American Cancer Society Research Professor of Pathology
UPMC Chair in Cancer Virology Research


Contact Information

Contact Information

Hillman Cancer Center
5117 Centre Ave
Suite 1.8
Pittsburgh, PA 15213

Email: yc70@pitt.edu

Administrative Assistant
Name: Bethany Darnley
Phone: 412-623-7941
Email: darnleyb@upmc.edu
Fax: 412-623-7715

Research Keywords

Research Keywords

Tumor viruses; Kaposi's sarcoma associated herpesvirus (KSHV); Merkel cell polyomavirus (MCV); digital transcriptome subtraction; tumor virus discovery

Research Summary

Research Summary

The work of our group (jointly directed by Patrick Moore and Yuan Chang) has focused on human tumor viruses since the early 1990s when we identified Kaposi’s sarcoma associated herpesvirus (KSHV/HHV8) and showed that this virus was causally associated with Kaposi’s sarcoma, the most common AIDS-related cancer in the United States and the most common malignancy in parts of Africa. We sequenced the KSHV genome, developed serologic assays, determined its prevalence in human populations, and characterized many of its critical viral oncoproteins. We have continued to study virus-host cell interactions in the context of dysregulation of pro-proliferative and anti-apoptotic pathways. We recently identified the seventh human tumor virus, Merkel cell polyomavirus (MCV), from a Merkel cell carcinoma (MCC). We characterized the transcriptional products of MCV and described the early region viral T antigen oncoproteins. Our work has established that MCV causes ~80% of MCC: we determined that the virus is clonally integrated in MCC tumor cells; that human tumor-associated Large T (LT) antigens contain signature truncation mutations; that T antigen proteins are expressed in MCC tumor cells by novel antibodies we developed; and we are the first laboratory to show rodent cell transformation by MCV sT antigen but not the LT antigen. We have identified several novel cellular interactors for MCV T antigens that open new avenues of investigating critical oncogenic signaling pathways. We have focused on many aspects of cancer etiology as modeled through oncogenic tumor viruses.

Selected Publications

Selected Publications

  • Chang Y, Cesarman E, Pessin M, Lee F, Culpepper J, Knowles DM, Moore PS. Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma. Science 1994; 266:1865-1869. PubMed Link
  • Moore PS and Chang Y. Detection of herpesvirus-like DNA sequences in Kaposi's sarcoma from persons with and without HIV infection. New Eng J Med 1995; 332:1181-85. PubMed Link
  • Chang Y, Moore PS, Talbot SJ, Boshoff C, Zarkowska T, Godden-Kent D, Paterson H, Weiss RA, Mittnacht S. Cyclin encoded by KS herpesvirus. Nature 1996; 382:410. PubMed Link
  • Moore PS, Boshoff C, Weiss RA, Chang Y. Molecular mimicry of human cytokine and cytokine-response pathway genes by KSHV. Science 1996; 274:1739-44. PubMed Link
  • Russo JJ, Bohenzky RA, Chien MC, Chen J, Yan M, Maddlena D, Parry JP, Peruzzi D, Edelman IS, Chang Y, Moore PS. The nucleotide sequence of Kaposi sarcoma-associated herpesvirus (HHV8). Proc Natl Acad Sci, USA 1996;10;93(25):14862-7. PubMed Link
  • Boshoff C, Endo Y, Collins PD, Takeuchi Y, Reeves JD, Schweickart VL, Siani M, Sasaki T, Williams TJ, Gray PW, Moore PS, Chang Y, Weiss RA. Angiogenic and HIV inhibitory functions of KSHV-encoded chemokines. Science 1997; 278:290-4. PubMed Link
  • Chatterjee M, Osborne J, Bestetti G, Chang Y, Moore PS. Viral IL-6-induced cell proliferation and immune evasion of interferon activity. Science 2002; 298:1432-5. PubMed Link
  • Feng H, Shuda M, Chang Y, Moore PS. Clonal integration of a polyomavirus in Merkel Cell Carcinoma. Science 2008; 319(5866): 1096-100. PubMed Link
  • Shuda M, Feng H, Kwun H, Rosen ST, Gjoerup O, Moore PS, Chang Y. T-antigen mutations are a tumor-specific signature for Merkel cell polyomavirus. Proc Natl Acad Sci, USA 2008; 21;105(42):16272-7. PubMed Link
  • Kwun HJ, Shuda M, Feng H, Camacho CJ, Moore PS, Chang Y. Merkel Cell Polyomavirus small T antigen controls viral replication and oncoprotein expression by targeting the cellular ubiquitin ligase SCFFbw7. Cell Host and Microbe 2013; 14(2):125-135. PubMed Link

See all pubs (Pubmed)

Collaborations

Collaborations

Current Internal Collaborators

Patrick Moore, MD, MPH
American Cancer Society Professor and Distinguished Professor of Microbiology and Molecular Genetics
Leader - Cancer Virology Program (CVP)

Shared Resource Usage

Shared Resource Usage

  • Animal Facility
  • Cell and Tissue Imaging Facility
  • Cytometry Facility
  • Tissue and Research Pathology Services

Affiliated Graduate Programs

Affiliated Graduate Programs

  • Cellular and Molecular Pathology
  • Molecular Virology & Microbiology
  • Program for Integrative Medical Biology

Department Website

Lab Website









Last modified: 6/2/14