University of Pittsburgh Cancer Institute (UPCI)

CCSG Acknowledgement

Required CCSG Acknowledgement

The NCI requires that publications acknowledge the UPCI CCSG support, and they are tracking compliance. If a UPCI CCSG-supported Shared Resource provided data used in your publication, please include the following statement in the acknowledgment section of your publication(s):

"This project used the UPCI [insert name(s) of shared resource(s)] that [is/are] supported in part by award P30CA047904."

Shared Resource Directors: Please make sure to include this statement on all of your order forms, contracts, etc. as a reminder to your users to acknowledge the UPCI CCSG support.


Fee Structure

To find out more about sequencing pricing, please contact Maureen Lyons or Patricia Petrosko.

Extraction and QA/QC Services

Extractions- Fresh and Frozen Tissue, FFPET, Cell Culture, Fluids

All extractions require QC via Bioanalyzer and UV Spectrophotometry via Nanodrop

RNA USD*
RNA Extraction Qiagen RNeasy Midi $41.00
RNA Extraction Qiagen RNeasy Mini $34.00
RNA Extraction Qiagen RNeasy Micro $35.00
RNA Extraction Qiagen miRNeasy $32.00
DNA
DNA Extraction Qiagen - Frozen Tissue or Whole Blood $21.00
Custom Paraffin Sample Extraction- 10um Slides or Scrolls
RNA Extraction from FFPET Samples- Qiagen RNeasy FFPET - Scrolls $38.00
RNA Extraction from FFPET Samples- Qiagen RNeasy FFPET - Slides $40.00
RNA Extraction from FFPET Samples- Qiagen miRNeasy FFPET - Scrolls $38.00
DNA Extraction Qiagen QIAamp (includes deparaffinization) $24.00
Macrodissection from FFPET slides (does not include deparaffinization or extraction) $38.00
Nucleic Acid Quality Analysis
Nucleic Acid Quantitation via nanodrop (DNA or RNA) $3.00
Nucleic Acid Quantitation (DNA or RNA)- QuBit $5.00
Bioanalyzer Services- Prices per chip, up to 12 samples
RNA Quality Analysis- Nano Chip $73.00
RNA Quality Analysis- Pico Chip $76.50
FFPET DNA Quality Analysis- 7500 Chip $75.00

*Cost per sample for 8+ samples



Expression Assays

miRNA Expression Service - Affymetrix miRNA Array Assay
Affymetrix miRNA Whole Assay (Labeling, Hybridization, Scan) $396.00 per sample
Affymetrix miRNA 4.0 Array $130.00 per sample
Total Cost per Sample for Affymetrix miRNA Assay $526.00


Expression Service - Affymetrix 3' Array Assay

For 3' Arrays (Affy HGU133 Plus 2.0, HGU133A 2.0, etc)

Ambion Labeling Assay
Ambion MessageAmp Premier Whole Assay (Labeling, Hybridization, Scan) $380.00 per sample
Affymetrix U133 Plus 2.0 Array (Whole Genome) $456.00 per sample
Total Cost per Sample for Affymetrix HGU133 Plus 2.0 Assay $836.00
Ambion MessageAmp Premier Whole Assay (Labeling, Hybridization, Scan) $380.00 per sample
Affymetrix U133A 2.0 Array $285.00 per sample
Total Cost per Sample for Affymetrix HGU133A 2.0 Assay $665.00
NuGen Ovation Assay - For samples with low starting input (500pg-50ng)
NuGen Ovation Pico Assay (Labeling, Hybridization, Scan) $442.00 per sample
Affymetrix U133 Plus 2.0 Array (Whole Genome) $456.00 per sample
Total Cost per Sample for Affymetrix HGU133 Plus 2.0 Assay $898.00
NuGen Ovation Pico Assay (Labeling, Hybridization, Scan) $442.00 per sample
Affymetrix U133A 2.0 Array $285.00 per sample
Total Cost per Sample for Affymetrix HGU133A 2.0 Assay $727.00
NuGen FFPET Assay- For FFPET Samples
NuGen Ovation FFPET Assay (Labeling, Hybridization, Scan) $406.00 per sample
Affymetrix U133 Plus 2.0 Array (Whole Genome) $456.00 per sample
Total Cost per Sample for Affymetrix HGU133 Plus 2.0 Assay $862.00
NuGen Ovation FFPET Assay (Labeling, Hybridization, Scan) $406.00 per sample
Affymetrix U133A 2.0 Array $285.00 per sample
Total Cost per Sample for Affymetrix HGU133A 2.0 Assay $691.00

Expression Service - Affymetrix Whole Transcript Array Assays

WT Expression Assays for Affy Gene ST and Exon ST Arrays

Ambion WT Expression Labeling Assay
Ambion WT Expression Assay (Labeling, Hybridization, Scan) $393.00 per sample
Affymetrix Human Exon 1.0 ST Array $456.00 per sample
Total Cost per Sample for Affymetrix Exon 1.0 ST Assay $849.00
Ambion WT Expression Assay (Labeling, Hybridization, Scan) $393.00 per sample
Affymetrix Human Gene 1.0 ST Array $200.00 per sample
Total Cost per Sample for Affymetrix Gene 1.0 ST Assay $593.00
NuGen WT Expression Assay
NuGen WT Expression Assay (Labeling, Hybridization, Scan) $332.00 per sample
Affymetrix Human Exon 1.0 ST Array $456.00 per sample
Total Cost per Sample for Affymetrix Exon 1.0 ST Assay $788.00
NuGen WT Expression Assay (Labeling, Hybridization, Scan) $332.00 per sample
Affymetrix Human Gene 1.0 ST Array $200.00 per sample
Total Cost per Sample for Affymetrix Gene 1.0 ST Assay $532.00
NuGen WT Expression Assay- FFPET Samples
NuGen WT Expression Assay- FFPET Samples $573.00 per sample
Affymetrix Human Exon 1.0 ST Array $456.00 per sample
Total Cost per Sample for Affymetrix Exon 1.0 ST Assay $1,029.00
NuGen WT Expression Assay- FFPET Samples $573.00 per sample
Affymetrix Human Gene 1.0 ST Array $200.00 per sample
Total Cost per Sample for Affymetrix Gene 1.0 ST Assay $773.00


Genotyping Services: Affymetrix


6.0 Assay
DNA Genotyping 6.0 Assay (Labeling, Hybridization, Scan) $436.00 per sample
Affymetrix Human Mapping 6.0 Array $342.00 per sample
Total Cost per Sample for Affymetrix Genotyping 6.0 Assay $778.00


Tiling Microarray Assay


Affymetric Promoter Tiling Assay**
DNA Promoter Array Assay* $421.00 per sample
Affymetrix Human Promoter 1.0R Array $375.00 per sample
Total Cost per Sample for Affymetrix Genotyping 250K STY Assay $796.00

**Researcher performs ChIP assay and provides CGF double-stranded, immunoprecipitated DNA: de-crosslinked and protein K treated



Other Services


Custom Array Scanning (Axon 4000B Scanner) $65.00
Data Analysis (per hour rate) $78.00
Training (per hour rate) $78.00
Equipment Usage (per hour rate) $78.00